Introduction

We create protocols for you, saving you significant time. We also don’t sell any supplements nor make any money from the interventions.

Integrative physicians often combine these metabolic interventions with lifestyle medicine, bioidentical hormones, advanced peptide therapy, and regenerative treatments to further bolster outcomes.

Click here to watch an overview of what Theriome provides.

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Background

A 64-year-old male patient underwent two rounds of comprehensive metabolomic testing through the Theriome Aristotle® Test. The first test provided baseline metabolic dysfunctions, while the second test evaluated improvements following a 6-month targeted intervention.

Initially, the patient presented with concerns related to systemic inflammation, cardiovascular health, cognitive function (brain fog), and longevity. After the first test, a personalized intervention protocol was implemented. The second test assessed the impact of this protocol, revealing significant improvements in key metabolic areas while identifying remaining areas for optimization. This dual-test approach provided quantifiable evidence of intervention success.

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Methodology

The patient underwent metabolomic profiling via the Theriome Aristotle® Test, analyzing 126 biomarkers across 12 key health domains. A systems biology approach and digital twin modeling were employed to generate a personalized intervention protocol, simulating 1,000 variations to determine the most effective strategy.

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Initial Findings

Baseline metabolic markers revealed dysfunctions in multiple Theriome domains, notably:

1. Aging Index: 56%
   – Signs of NAD+ depletion, oxidative stress, and mitochondrial inefficiency.

2. Inflammatory Index: 45%
   – Elevated cytokines (TNF-alpha, IL-6), excessive oxidative burden.

3. Mitochondrial Health: 42%
   – Impaired ATP production, inefficient electron transport chain activity.

4. Cardiovascular Health: 55%
   – Early indications of endothelial dysfunction and arterial stiffness.

5. Neurocognitive Index: 51%
   – Neurotransmitter imbalances contributing to brain fog and cognitive sluggishness.

6. Liver Health & Detoxification: 50%
   – Insufficient glutathione levels, signs of toxic burden.

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Personalized Protocol & Implementation

A targeted, systems-level intervention was developed to optimize metabolic function and address core dysfunctions. To ensure compliance, it is best to use a custom formula that consolidates multiple supplements into fewer capsules. We recommend Personalized Nutrients, where the physician can white-label customized formulas with a minimum order quantity of just one.

Key Components:

1. Mitochondrial Optimization

   • Nicotinamide riboside (NR) 300 mg
   • PQQ 20 mg
   • CoQ10 (Ubiquinol) 300 mg
   • D-Ribose 5 g daily
     
     

2. Inflammation Modulation

• • Curcumin 1,000 mg
  • Omega-3 (EPA/DHA 1.5 g)
  • Apigenin 50 mg at night

1. Cardiovascular Support

   • L-Arginine 3 g
   • Vitamin K2 (MK4 25 mg, MK7 0.5 mg)
   • Aged garlic extract 1,200 mg
     
     

2. Cognitive Function & Brain Health

   • Lion’s Mane 1,000 mg
   • CDP-Choline 250 mg
   • PharmaGABA 400 mg
     
     

3. Gut & Detoxification Support

   • Akkermansia probiotics 20B CFU
   • Tributyrin 350 mg
   • TUDCA 500 mg
   • Chlorella 1,000 mg
     
     

4. Environmental Toxin Reduction

   • Activated Charcoal (500 mg, 2x weekly)
   • Infrared sauna 3x per week

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Results After 6 Months

After six months of adherence to the personalized protocol, the patient demonstrated the following objective improvements in his biochemical profile:

• Aging Index: 56% → 60% (Improved NAD+ status, reduced oxidative stress markers)
• Inflammatory Index: 45% → 60% (Lower inflammatory cytokines, increased glutathione synthesis)
• Mitochondrial Health: 42% → 60% (Enhanced ATP production, reduced mitochondrial oxidative burden)
• Cardiovascular Health: 55% → 60% (Improved endothelial function and arterial elasticity)
• Cognitive Function: 51% → 60% (Better neurotransmitter balance, decreased brain fog)
• Liver Health & Detoxification: 50% (No improvement; indicates need for more robust detox interventions)

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Patient-Reported Outcomes

1. Energy & Mitochondrial Function

   • Noticeable midday energy improvements.
   • Reduced reliance on caffeine.
     
     

2. Cognitive Function & Brain Fog

   • Significant reduction in brain fog.
   • Better short-term memory and longer concentration span.
     
     

3. Inflammation & Joint Health

   • Reduced morning stiffness.
     

4. Cardiovascular & Circulatory Health

   • Better stamina and endurance for walking/physical activity.
   • No more cold extremities, suggesting improved peripheral circulation.
     
     

5. Sleep Quality & Recovery

   • Easier time falling asleep and staying asleep.
     
     

6. Areas Needing Further Attention

   • Liver health showed no significant improvement.
   • Mild lingering inflammation remains.
   • Goal to further enhance endurance and strength.

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How we Create Protocols with Digital Twin Simulations

Prior to adjusting interventions, we run 1,000 simulated variations accounting for:

• AI-based metabolic simulations
• Metabolomic modeling
• Clinical response patterns from prior improvements

Example: If CoQ10 levels improved but mitochondrial function only modestly increased, this might indicate inefficient ATP synthesis, an electron transport chain bottleneck, or persistent oxidative stress. Instead of merely increasing CoQ10, we might:

• Check carnitine status to optimize fat metabolism
• Add D-Ribose + PQQ for ATP synthesis
• Increase mitochondrial antioxidants like Astaxanthin or Alpha-Lipoic Acid

This process is repeated systematically to capture non-linear biological interactions that might otherwise be missed.

Determining the most impactful interventions

Not every intervention is equally impactful. We focus on:

1. Effect Size – Which interventions statistically yield the largest improvement.
2. Metabolite Sensitivity – Some biomarkers respond faster to changes than others.
3. Biological Adaptation Speed – Mitochondrial biogenesis is a longer-term adaptation compared to short-term inflammatory modulation.

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Retester Analysis: Initial Validation of our Protocols

Our Retester Analysis represents an early yet promising validation of the personalized protocols we develop. We conduct regular retesting of individuals using:

• Theriome Aristotle® Test (126 metabolomic biomarkers)
• DXA for body composition
• VO₂max testing for cardiorespiratory fitness
• Lymphocyte panel for immune function insights

Early findings indicate that individuals who follow their personalized plans with 65% compliance or greater experience measurable improvements.

I.E. If you follow 2/3 of a protocol under a specific domain (such as neurocognitive function), you should see a significant improvement in 4 months on average.

We intend to publish full results later this year, including detailed analyses of intervention efficacy and longitudinal outcomes. 

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Conclusion

To get started with Theriome in your clinic, click this link: www.therio.me/train

See a sample report.